Validating the genomic signature of pediatric septic shock Web sex no charge
Circulating blood cells from critically ill patients with septic shock exhibit decreased oxidative respiration, electron chain complex activity, mitochondrial turnover, and mitochondrial membrane potential .Blood is an easily accessible tissue that can be used to directly measure mitochondrial dysfunction in immune cells and may reflect a systemic process affecting other vital organs.
Total RNA was isolated from whole blood using the Pax Gene Blood RNA System (Pre Analyti X, Qiagen/Becton Dickson, Valencia, CA, USA).
Image files were captured using an Affymetrix Gene Chip Scanner 3000. CEL) were subsequently preprocessed using robust multiple-array average (RMA) normalization and Gene Spring GX 7.3 software (Agilent Technologies, Palo Alto, CA, USA).